Our lab is interested in the following questions:

(1) What kinetochore proteins form the binding sites for the plus-end of spindle microtubules? How do the proteins coalesce to form these sites?
(2) What proteins build the platform for the microtubule binders?
(3) How do kinetochores detach from microtubules to correct errors in attachment?
(4) How is the strength of kinetochore-microtubule attachments regulated to allow for fluid chromosome movements?
(5) Does mis-regulation of the binding strength between kinetochores and microtubules lead to aneuploidy and cancer? What proteins are implicated in this?

Our lab uses a variety of approaches to attack these questions including:

-gene silence and rescue assays in cultured cells
-quantitative laser-based microscopy assays including FRAP (fluorescence recovery after photobleaching), FRET (fluorescence resonance energy transfer), BiFC (bimolecular fluorescence complementation), and PA (photoactivation)
-live-cell time-lapse microscopy
-in vitro biochemical assays
-yeast two hybrid assays
-super-resolution light microscopy